ARMC10 regulates mitochondrial dynamics and affects mitochondrial function via the Wnt/β‐catenin signalling pathway involved in ischaemic stroke

Abstract Mitochondrial dynamics has emerged as an important target for neuronal protection after cerebral ischaemia/reperfusion. Therefore, the aim of this study was to investigate the mechanism by which ARMC10 regulation of mitochondrial dynamics affects mitochondrial function involved in ischaemic stroke (IS). Mitochondrial morphology was detected by laser scanning confocal microscopy (LSCM), and mitochondrial ultrastructural alterations were detected by electron microscopy. The expression of mitochondrial dynamics‐related genes Drp1, Mfn1, Mfn2, Fis1, OPA1 and ARMC10 and downstream target genes c‐Myc, CyclinD1 and AXIN2 was detected by RT‐qPCR. Western blot was used to detect the protein expression of β‐catenin, GSK‐3β, p‐GSK‐3β, Bcl‐2 and Bax. DCFH‐DA fluorescent probe was to detect the effect of ARMC10 on mitochondrial ROS level, Annexin V‐FITC fluorescent probe was to detect the effect of ARMC10 on apoptosis, and ATP assay kit was to detect the effect of ARMC10 on ATP production. Mitochondrial dynamics was dysregulated in clinical IS samples and in the OGD/R cell model, and the relative expression of ARMC10 gene was significantly decreased in IS group (p < 0.05). Knockdown and overexpression of ARMC10 could affect mitochondrial dynamics, mitochondrial function and neuronal apoptosis. Agonist and inhibitor affected mitochondrial function and neuronal apoptosis by targeting Wnt/β‐Catenin signal pathway. In the OGD/R model, ARMC10 affected mitochondrial function and neuronal apoptosis through the mechanism that regulates Wnt/β‐catenin signalling pathway. ARMC10 regulates mitochondrial dynamics and protects mitochondrial function by activating Wnt/β‐catenin signalling pathway, to exert neuroprotective effects.


| INTRODUC TI ON
10] The fission and fusion of mitochondria within the cell are known as mitochondrial dynamics and are controlled by a series of proteins. 11,12Several genes controlling mitochondrial fission/fusion dynamics have been identified, such as fission protein 1 (Fis1), mitochondrial fission factor (Mff), dynamic-related protein 1 (Drp1) and mitofusin 1/2 (Mfn1/2).Generally, Drp1 and Fis1 control mitochondrial fission, while Mfn1/2, Optic Atrophy 1(OPA1) control mitochondrial fusion.[15] In ischaemic stroke, inadequate oxygen and nutrient supply disrupts energy homeostasis and adenosine triphosphate (ATP) synthesis, ultimately leading to mitochondrial dysfunction and permanent brain damage.Many studies have found that mitochondrial dynamics play an important role in ischaemic stroke.
For example, Liu et al. noted that hyperglycaemia exacerbates cerebral ischaemia/reperfusion-induced neuronal injury by activating cellular autophagy and mitochondrial division via ERK1/2. 16 et al. investigated that ligustilide induces Drp1-mediated mitochondrial fragmentation in vivo and in vitro through activation of the AMPK signalling pathway, protecting against neural injury and preventing ischaemic stroke. 17 exacerbates neuronal mitochondrial fragmentation and injury and participates in neuronal ischaemia-reperfusion injury in a GTPase-dependent manner. 18e armadillo repeat containing 10 (ARMC10) gene, also known as splicing variant involved in hepatocarcinogenesis (SVH), is located on chromosome 7 and encodes the ARMC10 (SVH) protein.Different splice variants can form four isoforms of SVH-A, B, C and D proteins, which can be expressed in a variety of tissues and are particularly highly expressed in human brain and kidney. 19Serrat et al. found that ARMC10 have unique features in the functional regulation of mitochondrial dynamics. 20Jan Kriska noted that the Wnt/β-catenin signalling pathway promotes neurogenesis in neural stem/progenitor cells and activates neuronal differentiation in the subventricular zone. 21However, there are few reports on the involvement of ARMC10 in the pathological process and mechanism of ischaemic stroke.Therefore, the aim of the present study was to investigate the role of ARMC10 through the altering mitochondrial dynamics involved in the pathological process of ischaemic stroke, thereby affecting neuronal function.
In the present study, we firstly investigated the correlation between mitochondrial dynamics and IS, and then, we constructed ARMC10 knockdown and overexpression cell models to confirm that ARMC10 regulates mitochondrial dynamics and thus affects mitochondrial function and neuronal apoptosis.Next, we detected the expression levels of key molecules and downstream target genes of the Wnt/β-catenin signalling pathway, to explore the mechanism of ARMC10 regulating mitochondrial function involved in IS neuronal injury.Finally, OGD/R model was constructed in SH-SY5Y cells to verify the hypothesis.

| Cell transfection
The overexpression plasmid vector of ARMC10 constructed by Hippobio (Zhejiang, China).SH-SY5Y cells were cultured in six-well plates at a density of 6 × 10 5 cells per well and transfected with siRNA and overexpression plasmid of ARMC10 gene, Ribo FECT™ CP (RIBBIO, China) and Lipofectamine™ 3000 Transfection Reagent (ThermoFisher, America) were used in accordance with the manufacturer's protocol.
Follow-up experiments were carried out after 48 h of culture.
2.2.3 | Oxygen-glucose deprivation/re-oxygenation (OGD/R) treatment SH-SY5Y cells were seeded in 6-well plate and perform OGD/R processing when the density reaches 80%.Discarded the culture medium, washed the cells gently with PBS and add sugar-free DMEM medium (BI, Israel) (serum-free) and placed in 37°C anaerobic incubator for 24 h.Subsequently, the medium was changed to normal medium and cultured in CO 2 incubator for 8 h.CCK-8 Kit (Solarbio, China) was used to detect cell viability of different time points, OGD24 h/R8 h was selected to establish OGD/R model.Each group was provided with 3 wells.

| RT-qPCR
Refer to the Hieff qPCR SYBR Green Master Mix manual (YEASEN, China) to prepare the RT-qPCR reaction system and set the reaction conditions.The primers were used for RT-qPCR were synthesized by Tsingke Biotechnology Company (China).Relative RNA levels detected by RT-qPCR using the SYBR Green method.The relative expression of RNAs was normalized to β-actin/β-catenin mRNA calculated using the comparative CT method.Primer sequences were shown in Table 1.

Gene Primers
determination kit (Solarbio, China) determine the concentration of total protein.Boil samples 10 min and immediately put on the ice.
According to the molecular weight of the target protein, prepared PAGE Gel (Epizyme Biotech, China).Fully separate protein through electrophoresis, then transferred onto PVDF membranes and blocked with 5% skim milk powder.The following protein level were detected by Image information was collected with ultra-sensitive exposure system.

| ATP assay
Mitochondrial ATP content was detected using ATP Assay Kit (Beyotime Biotechnology, China).Remove culture medium and add 200 μL lysis buffer, vortex to lyse fully, centrifuged with cryogenic centrifuge at 12,000×g for 5 min.Take the supernatant for future experiment.Add 100 μL ATP detection working fluid to the detection hole and place 5 min at room temperature.Add 50 μL standard solution or sample in per hole then luminescence was detected with CentroLB960 Micro-orifice plate luminescence detector.

| Statistical analysis
The images of western blot were processed by ImageJ software, and the experimental data were statistically analysed by GraphPad Prism 8.0.1.All the experimental data were tested for normality and homogeneity of variance, and then, independent sample t-test or one-way analysis of variance (one-way ANOVA) was performed.The statistical results were expressed in the form of mean ± standard deviation (mean ± SD), α = 0.05, p < 0.05 indicated that the difference was statistically significant.

| Changes of mitochondrial dynamics and function in clinical samples and OGD models
To explore the correlation between ischaemic stroke and mitochondrial dynamics, the peripheral blood of three IS patients and three healthy controls were collected to extract mononuclear cells, and then, the morphological characteristics of mitochondria were observed with LSCM.The results showed significant fragmentation of mitochondria in IS group.The mitochondrial aspect ratio (AR) value was significantly lower than that in control, while the mitochondrial fragmentation count (MFC) and sphericity results were opposite to the AR trend.(Figure 1A).
The in vitro experimental results also showed that OGD/R treatment had an impact on mitochondrial morphology.The value of form factor (FF) in OGD/R group was significantly lower, while the MFC value of the OGD/R group was significantly higher than control (Figure 1B).After OGD/R, the ultrastructure of mitochondria underwent significant changes, with a significant increase in the proportion of swollen mitochondria and a significant decrease in the proportion of mitochondria with more than 5 cristae (Figure 1C).
Mitochondrial dysfunction occurs after OGD/R treatment, characterized by an increase in mitochondrial ROS levels and a decrease in ATP production.In addition, the apoptosis rate of SHSY-5Y cells in the OGD/R group significantly increased, indicating that OGD/R treatment increased neuronal apoptosis (Figure 1D).

| Exploring genes that regulate mitochondrial dynamics changes
In order to further explore the genes that regulate mitochondrial dynamics changes, RT-qPCR was used to detect the expression of mitochondrial dynamics-related genes in the IS group and the control group.The results showed that the relative expression level of ARMC10 in IS group was significantly lower than that in normal control (p < 0.01), but there was no significant difference in the expression level of Drp1, Mfn1, Mfn2, Fis1 and OPA1 between the two groups (p > 0.05) (Figure S1A).Consistent with the clinical samples, the results of RT-qPCR and western blot showed that ARMC10 expression is significantly reduced after ischaemia-reperfusion (I/R) injury (Figure 2B,C; Figure S1B).

| Verify the effect of ARMC10 on mitochondrial dynamics
Mitochondrial morphology analysis showed that knocking down ARMC10 resulted in significant fragmentation of mitochondrial morphology.The AR value significantly decreased, while the sphericity and MFC value significantly increased.While overexpression of ARMC10 could reduce mitochondrial disruption caused by OGD/R (Figure 3A).In addition, the ultrastructure

| ARMC10 affects mitochondrial function and Neuronal apoptosis
Then, we validated that the change of ARMC10 expression level affected mitochondrial function and neuronal apoptosis.Transfection of si-ARMC10 significantly increased ROS production and decreased ATP production, while overexpression of ARMC10 significantly decreased ROS content and increased ATP production (Figure 4A).In addition, silencing ARMC10 could up-regulate the expression of proapoptotic protein Bax and reduce the level of anti-apoptotic protein Bcl-2, while overexpression of ARMC10 could lead to the opposite result (Figure 4B).The results of flow cytometry were consistent with western blot, indicating a significant rise in cell apoptosis in si-ARMC10 group.The cell apoptosis rate of OGD/R + Empty plasmid group was significantly higher, and overexpression of ARMC10 could rescue OGD/R induced cell apoptosis (Figure 4C).

| ARMC10 activates Wnt/β -Catenin signal pathway and initiates the expression of downstream target genes
Next, we explored whether ARMC10 activate Wnt/β-Catenin signalling pathway by which ARMC10 affects mitochondrial function and leads to neuronal apoptosis.In SH-SY5Y cells, the expression levels of ARMC10 were altered by transfection of si-ARMC10, ARMC10 overexpression plasmids and pCDH empty plasmids, respectively.
We hypothesized that knockdown of ARMC10 affected key proteins of Wnt/β-catenin signal pathway, including β-catenin, GSK-3β and p-GSK-3β, which have been reported. 22,23Western blot and RT-qPCR confirmed that the expression of β-catenin and p-GSK-3β decreased significantly, while the expression level of GSK-3β increased significantly.In addition, the results of western blot and RT-qPCR showed that the overexpression of ARMC10 up-regulated the level of βcatenin and p-GSK-3β, and the expression of GSK-3β was reduced at the same time (Figure 5A; Figure S2A).decreased significantly, while after overexpression of ARMC10, the decrease of β-catenin transformation was rescued (Figure 5D).
After Wnt/ β-catenin signalling pathway was activated, β-catenin transferred from cytoplasm to nucleus, initiating transcription and translation of downstream target genes. 24,25Knocking down ARMC10 significantly up-regulated the expression of AXIN2 at both mRNA and protein level, while the expression of AXIN2 decreased significantly after overexpression of ARMC10 (Figure 5B).The expression of c-Myc and CyclinD1 significantly decreased in si-ARMC10 group and up-regulated the expression of c-Myc and CyclinD1 after overexpression of ARMC10 (Figure 5C).and the up-regulation of GSK-3β induced by OGD/R (Figure 7A;

| Agonist and inhibitor affect mitochondrial function and neuronal apoptosis by targeting Wnt/β -catenin signal pathway
Figure S2C).The results of RT-qPCR and western blot showed that β-catenin nuclear translocation was reduced and AXIN2 expression was increased, while cMyc and CyclinD1 expression was decreased after OGD/R treatment, but the above effects were reversed when ARMC10 was overexpressed (Figure S3).
Compared with the control group, the OGD/R group showed a significantly increasing in ROS generation and a significantly decreasing in the level of ATP generation.Relative to the OGD/R + Empty group, OGD/R + OE group showed that ROS production was significantly decreased and ATP production level was significantly increased; OGD/R + LiCl group showed a significantly inhibited in ROS production and a significantly rise in ATP production level compared with OGD/R group (Figure 7B).
The expression of apoptotic protein Bax protein was significantly up-regulated and the expression of anti-apoptotic protein Bcl-2 protein was significantly down-regulated in the OGD/R group compared with the control group; the expression of Bax protein was significantly down-regulated and the expression of Bcl-2 protein was significantly up-regulated in the OGD/R + ARMC10-OE group relative to the OGD/R + Empty group.The results are shown in Figure 7C.

| DISCUSS ION
Mitochondrial dynamics, a key mechanism for mitochondrial quality control, has emerged as an important target for neuronal protection after cerebral ischaemia/reperfusion. 26We found that mitochon- on mitochondrial dynamics in an adult male rat model of stroke and found that OPA1 was up-regulated and cerebral oedema was reduced after cerebral ischaemia. 27This was consistent with our findings.Ischaemic stroke induces an increase in mitochondrial fission, which contributes to increased mitochondrial energy production.
However, excessive fission impairs normal mitochondrial function.function. 30,31ATP synthesis and ROS production are often used as important indicators for evaluating mitochondrial function. 32,33r results showed that transfection of si-ARMC10 significantly increased ROS production and decreased ATP production, whereas ischaemia/reperfusion injury. 34Mirra S et al found that ARMC10 is expressed in chicken spinal cord nerve and participates in the regulation of chicken spinal cord nerve development by regulating Wnt/β-catenin signal pathway. 35Therefore, we hypothesized that ARMC10 may be involved in cerebral ischaemia-reperfusion injury by regulating the Wnt/β-catenin signalling pathway.
We examined the expression levels of key molecules (β-catenin, GSK-3β, p-GSK-3β) and downstream genes (c-Myc, CyclinD1, AXIN2) of the Wnt/β-catenin signalling pathway, and our results showed that overexpression of ARMC10 activated the Wnt/β-catenin signalling pathway, while knockdown of ARMC10 could inhibit the activity of Wnt/β-catenin signalling pathway.The transfer level of β-catenin from cytoplasm to nucleus was crucial step that Wnt/β-catenin signalling pathway plays an important role in biological functions.
We found that knockdown of ARMC10 significantly reduced the amount of β-catenin protein in the nucleus, whereas overexpression of ARMC10 significantly increased the amount of β-catenin protein in the nucleus, which further confirmed that ARMC10 could activate the Wnt/β-catenin signalling pathway.In addition, we further explored the relationship between the Wnt/β-catenin signalling pathway and mitochondrial function and neuronal apoptosis using

| CON CLUS ION
In conclusion, our study demonstrates that ARMC10 regulates mitochondrial dynamics and affects mitochondrial function through the Wnt/β-catenin signalling pathway involved in cerebral Li et al. expounded that OMA1-mediated OPA1 cleavage (S1-OPA1) and then S1-OPA1

Fifty-three patients 2 . 2 |
with first-ever IS who attended the First People's Hospital of Zhengzhou City from October 2018 to February 2019 were included in this study, while 53 individuals who underwent health examination at the First Affiliated Hospital of Henan University of Traditional Chinese Medicine were randomly selected as controls.The selected patients had to be admitted to the hospital within 2 days after the occurrence of stroke.IS was diagnosed according to the diagnostic criteria revised by the Fourth National Academic Conference on Cerebrovascular Disease.Those with family history of diabetes mellitus, cardiovascular disease and hypertension were excluded.All study subjects were Han Chinese and not related to each other by blood.The study protocol was approved by the Ethical Committees of Zhengzhou University.All study participants signed an informed consent form.Cell transfection and oxygen-glucose deprivation/re-oxygenation (OGD/R) treatments 2.2.1 | Cell culture SH-SY5Y, a neuroblastoma cell line, Cells were purchased from National Collection of Authenticated Cell Cultures.SH-SY5Y was cultured with MEM/F-12 (1:1, Hyclone, China) containing 10% foetal bovine serum (FBS, BI, Israel) and 1% Streptomycin mixture (Solarbio, China).When the cell density reaches more than 80%, it can be subcultured.Washed cell with PBS twice and digested with 1 mL trypsin-EDTA for 2 min.Collected cells after termination of digestion, centrifuge at 1000 rpm for 5 min.Resuspension cell and placed in a 25 T culture flask bottle, culture it in a constant temperature incubator.Culture conditions: 5% CO 2 , 37°C, humidity 95%.

2. 3 |
Extraction and purification of DNA and RNA, RNA reverse transcription 2.3.1 | Extraction of peripheral blood PBMCs Transfer blood from EDTA-Na 2 tube to 15 mL centrifuge tube, adding equal volume of physiological saline.Mixed with 3 mL of human peripheral blood mononuclear cell isolation solution, centrifuge at 2000 rpm for 25 min.Suck out the leukocytes and transfer to new 15 mL centrifuge tube, five-fold volume of PBS solution suspend cells, centrifuge at 1000 rpm for 5 min, abandon supernatant and add 2 mL of 1640 complete medium for Mitotracker Red staining.

2. 3 . 2 |
RNA extraction and RT-qPCR Cells were collected and mixed with 1 mL Trizol and left on ice 5 min.Add 0.2 mL chloroform per 1 mL Trizol and place 3 min on ice.Removed upper water phase into new tube after 4°C 12,000×g centrifugal 15 min, mixed with 500 μL isopropyl alcohol and set on ice 10 min and centrifuged again.Used 75% ethanol wash subside twice, dry it and add RNase-free water.Detect RNA by NanoDrop 2000 spectrophotometer.First-strand cDNA was synthesized using Reverse transcriptase kit (YEASEN, China).

2. 4 . 1 |
Nuclear/cytoplasmic separation protein extraction Cytoplasmic protein extraction reagents A and B were added, respectively, vortex to fully disperse the cell precipitation, ice bath.Centrifugation at 12,000-16,000×g for 5 min at 4°C, the supernatant obtained was cytoplasmic protein, transferred to a new EP tube and temporarily placed on ice.Cytoplasmic protein extraction reagent was added into precipitate, vortex 15 s per 15 min, centrifuged again after 40 min.Remove supernatant into new tube, that is, the nuclear protein.Total protein was extracted with RIPA buffer(high) (Solarbio, China) and PMSF (Solarbio, China) using BCA protein concentration TA B L E 1 Primer sequences of RT-qPCR.

Intracellular
ROS was measured by means of DCFH-DA fluorescent probe (Reactive Oxygen Species Assay Kit, Beyotime Biotechnology, China).Cells were collected and washed with PBS and added 1 mL diluted DCFH-DA; positive control group was added with 1 mL diluted group.After incubate cells in 37°C incubator 20 min, wash cells with PBS three times and detect DCF on the flow cytometry (BD, America).Group was added to the positive control wells.2.5.3 | Apoptosis assaysApoptosis was evaluated using the Annexin V-FITC/PI Apoptosis Detection kit(Beyotime Biotechnology, China).Collected cells and washed with PBS, and resuspended in 500 μL binding buffer.10 × 10 4 suspended cells were centrifuged by 1300 rpm for 5 min, the supernatant was discarded and mixed with 195 μL Annexin V-FITC binding solution.Add 5 μL Annexin V-FITC and 10 μL propidium iodide (PI) staining solution in turn and mix gently.Incubate 20 min without light at room temperature and shake every other 5 min during the period to improve dyeing efficiency.Cold storage away from light, flow detection was completed within 1 h.

1
Changes of mitochondrial dynamics and function in clinical samples and OGD models (*p < 0.05, **p < 0.01, ***p < 0.001).(A) The mitochondrial morphology of PBMCs was compared between IS group and control group with LSCM (Mitotracker Red was used for mitochondrial staining).(B)Comparison of mitochondrial morphology between control group and OGD/R group (Mitotracker Red was used for mitochondrial staining).(C) The changes of mitochondrial ultrastructure after OGD/R treatment were observed by electron microscopy.(D) After OGD/R, flow cytometry was used to detect the level of ROS and apoptosis, ATP detection with Microplate Luminometer.ofmitochondria also changed significantly after knockdown of ARMC10, showing that the proportion of swollen mitochondria increased significantly and the number of crista decreased significantly.Compared with OGD/R + ARMC10 Empty, the proportion of mitochondrial swelling was significantly reduced, and the number of crista was significantly increased after overexpression of ARMC10 (Figure3B).
The transfer level of β-catenin from cytoplasm to nucleus is crucial step that Wnt/β-catenin signalling pathway plays an important role in biological functions.Therefore, SH-SY5Y cells were collected for nucleocytoplasmic separation and extraction of nuclear protein, and then, the transfer level of β-catenin protein from cytoplasm to nucleus was detected by western blot.After knocking down ARMC10, the amount of β-catenin transferred from cytoplasm to nucleus F I G U R E 2 Exploring genes that regulate mitochondrial dynamics changes.(*p < 0.05, **p < 0.01, ***p < 0.001).(A) RT-qPCR detect the expression of ARMC10 in peripheral blood of the control group and the IS group.(B) RT-qPCR detect ARMC10 expression levels of SH-SY5Y in control group and OGD/R group.(C) ARMC10 expression levels of SH-SY5Y in ODG/R group and control group.F I G U R E 3 Verify the effect of ARMC10 on mitochondrial dynamics (*p < 0.05, **p < 0.01, ***p < 0.001).(A) Effect of ARMC10 expression on mitochondrial morphology was observed by LSCM (Mitotracker Red was used for mitochondrial staining).(B) The effect of ARMC10 expression on mitochondrial ultrastructure was observed by electron microscopy.
LiCl and XAV-939 are commonly used agonist and inhibitor targeting the Wnt/β-catenin signal pathway.Therefore, LiCl and XAV-939 were used to detect their effects on mitochondrial function and neuronal apoptosis.Protein expression levels of βcatenin, GSK-3β and p-GSK-3β were detected by western blot, and the results showed that LiCl treatment significantly up-regulated the protein expression of β-catenin and p-GSK-3β and inhibited the expression of GSK-3β; compared with DMSO, the protein expression of β-catenin and p-GSK-3β was down-regulated and the expression of GSK-3β was significantly increased in XAV-939 group (Figure6A; FigureS2B).Expression level of CyclinD1 and c-Myc increased significantly by treatment with LiCl, XAV-939 could down-regulate the level of CyclinD1 and c-Myc (Figure6B).The application of LiCl attenuated the increasing of ROS production and decreasing of ATP production induced by ARMC10 silencing.The use of pathway inhibitors showed the same effect (Figure6C).After LiCl treatment, the expression level of pro-apoptotic protein Bax was significantly down-regulated, and the expression level of anti-apoptotic protein Bcl-2 was significantly up-regulated, and opposite results after the treatment of XAV-939 were observed (Figure6D).

F I G U R E 4 3 . 7 |
ARMC10 affects mitochondrial function and Neuronal apoptosis (*p < 0.05, **p < 0.01, ***p < 0.001).(A) Flow cytometry detect the effect of ARMC10 expression on mitochondrial function.(B) Western blot detect the effect of ARMC10 expression on apoptosis proteins.(C) Flow cytometry was used to detect the effect of ARMC10 expression on cell apoptosis.In OGD/R model, ARMC10 regulates Wnt/β -catenin signalling pathway affecting mitochondrial function and neuronal apoptosis Finally, we validated that ARMC10 activates Wnt/β-catenin pathway and affects mitochondrial function and neuronal apoptosis in OGD/R cell model.Under the condition of OGD/R, the expression of β-catenin and p-GSK-3β decreased significantly, while the expression of GSK-3β increased significantly, and the overexpression of ARCM10 rescued the down-regulation of β-catenin and p-GSK-3β drial dynamics were dysregulated in clinical IS samples and in the OGD/R cell model, mitochondrial morphology of PBMCs from IS patients exhibited significant fragmentation changes.Similarly, we observed mitochondrial fragmentation, mitochondrial swelling and F I G U R E 5 ARMC10 activates Wnt/β-Catenin signal pathway and initiates the expression of downstream target genes (*p < 0.05, **p < 0.01, ***p < 0.001).(A) RT-qPCR and western blot detected protein expression levels of key molecules in Wnt/β-catenin signalling pathway.(B) RT-qPCR and western blot detected expression of AXIN2.(C) RT-qPCR and western blot to determine the changes of downstream target genes of Wnt/β-catenin signalling pathway under different conditions.(D) The level of β-catenin protein in the nucleus was detected by nucleoplasm separation.a reduction in cristae in the OGD/R cell model.The ultrastructure of mitochondria changed markedly after OGD/R: the proportion of swollen mitochondria increased markedly, and the proportion of mitochondria with cristae more than five decreased markedly.Zhang et al. investigated the effects of 2-week exercise preconditioning Dysfunctional mitochondrial dynamics can impair mitochondrial distribution and transport in neurons, thereby shortening energy supply and inducing apoptosis.Restoring the balance between mitochondrial fusion and fission is beneficial for recovery from ischaemic stroke.Mitochondrial dynamics are recognized as a potential therapeutic target for ischaemic stroke.So, which genes regulate mitochondrial dynamics and influence mitochondrial function during the pathology of ischaemic stroke?Chen et al. found that in the transient global cerebral ischaemia model of rat, hypoglycaemia and hypoxia can depress the phosphorylation of Drp1 at Ser616 in the CA1 region of the hippocampus, which leads to increased mitochondrial division.28Li et al. constructed a Glucose-Oxygen Deprivation/Reperfusion (OGD/R) model in the PC-12 cell line and found that the expression of the mitochondrial fusion gene Mfn2 was reduced, and the mitochondrial morphology was fragmented. 29Therefore, several genes which control mitochondrial fission/fusion dynamics such as Drp1, Mfn1, Mfn2, F I G U R E 6 Agonist and inhibitor affect mitochondrial function and neuronal apoptosis by targeting Wnt/β-Catenin signal pathway (*p < 0.05, **p < 0.01, ***p < 0.001).(A) Western blot detected protein expression levels of key molecules in Wnt/β-catenin signalling pathway.(B) Western blot was used to detect the expression of downstream target genes of Wnt/β-catenin signalling pathway.(C) FCM was used to detect effects of LiCl (20 mM) and XAV-939 (10 μM) on mitochondrial function.(D) Western blot was used to detect the effect of ARMC10 expression on apoptosis proteins.Fis1, OPA1 and ARMC10 were selected.The results showed that the relative expression level of ARMC10 in IS patients was significantly lower than that in normal control (p < 0.01), but there was no significant difference in the expression levels of Drp1, Mfn1, Mfn2, Fis1 and OPA1 between the two groups (p > 0.05).Based on literature review, there was little relevant study on the relationship between ARMC10 and ischaemic stroke currently.ARMC10, as a gene associated with mitochondrial dynamics that may be involved in the pathologic process of IS, has attracted our attention and interest.First of all, we explored whether ARMC10 affects mitochondria dynamic.Morphology analysis showed that knockdown of ARMC10 resulted in significant fragmentation of mitochondrial morphology, the AR value was significantly reduced, while the sphericity and MFC value were significantly increased.In addition, overexpression of ARMC10 reduced mitochondrial disruption.In addition, the ultrastructure of mitochondria also changed significantly after knockdown of ARMC10, which was manifested as a significant increase in the proportion of swollen mitochondria and a significant decrease in the number of crista.Overexpression of ARMC10 resulted in a significant decrease in the proportion of swollen mitochondria and a significant increase in the number of cristae.In conclusion, knockdown of ARMC10 in SH-SY5Y cells significantly impaired mitochondrial dynamics, while overexpression of ARMC10 rescued the impairment of mitochondrial dynamics.Mitochondrial dynamics is an important mechanism for maintaining mitochondrial homeostasis and is closely related to mitochondrial F I G U R E 7 In OGD/R model, ARMC10 regulates Wnt/β-Catenin signalling pathway affecting mitochondrial function and neuronal apoptosis (*p < 0.05, **p < 0.01, ***p < 0.001).(A) The key molecules of Wnt/β-catenin signalling pathway were detected by western blot.(B) FCM was used to detect the function of different groups of mitochondria.(C) Expression of apoptosis-related proteins under different conditions.(A and C shares the same batch of GAPDH bands in their WB results) overexpression of ARMC10 significantly decreased ROS content and ATP production.Mitochondrial fusion/fission dynamics broadly affect neuronal function, including neuronal survival and plasticity.Mitochondrial dysfunction can cause apoptosis through the release of Cytochrome-c (Cyt-C) or pro-apoptotic proteins.Our results showed that silencing ARMC10 could up-regulate the expression of pro-apoptotic protein Bax and reduce the level of anti-apoptotic protein Bcl-2, while overexpression of ARMC10 can lead to the opposite result.The cell apoptosis rate of OGD/R + Empty plasmid group was significantly higher, and overexpression of ARMC10 could rescue OGD/R induced cell apoptosis.Serrat et al. found that ARMC10 protein was localized in the outer mitochondrial membrane by immunofluorescence staining methods and molecular labelling techniques.In addition, an Alzheimer's disease (AD) model was constructed in mouse neuronal cells, and it was found that overexpression of ARMC10 reduced the number of mitochondria in neuronal cells in the exercise state and promoted the occurrence of mitochondrial aggregation; at the same time, overexpression of ARMC10 rescued mitochondrial fragments, reduced β-amyloid (Aβ)-induced neuronal apoptosis and exerted neuroprotective effects. 20This is consistent with our findings.From this, we concluded that ARMC10 protein is localized in the outer mitochondrial membrane, and overexpression of ARMC10 can promote mitochondrial fusion and rescue OGD/R injury-induced mitochondrial fragmentation and mitochondrial ultrastructure destruction, thereby protecting mitochondrial function and attenuating neuronal apoptosis.The Wnt/β-catenin signal pathway participates in the regulation of developmental processes and CNS mutation, including the proliferation, differentiation and migration of neuronal cells, axon growth and synaptogenesis, and plays an important role in inhibiting cell apoptosis and promoting cell survival.Liu et al. showed that enhancement of the Wnt/β-catenin signalling pathway could protect hepatic mitochondrial function, reduce mitochondria-mediated endogenous hepatocyte apoptosis and ultimately attenuate hepatic inhibitors and agonists of the β-catenin signalling pathway, and the results showed that activation of the Wnt/β-catenin signalling pathway could protect mitochondrial function and reduce neuronal apoptosis.Inhibition of the Wnt/β-catenin signalling pathway can lead to mitochondrial dysfunction and promote neuronal apoptosis.Finally, we constructed an OGD/R cell model using SH-SY5Y cell line to further validate the role of ARMC10-dependent Wnt/β-catenin signalling pathway in brain I/R injury.We examined the expression levels of key molecules and downstream genes of the Wnt/β-catenin signalling pathway, as well as mitochondrial function and neuronal apoptosis.The results showed that OGD/R treatment inhibited the activation of the Wnt/β-catenin signalling pathway and decreased the expression of downstream target genes, while overexpression of ARMC10 had a rescue effect.In addition, we found that OGD/R treatment induced a decrease in ATP production and an increase in ROS production, aggravating neuronal apoptosis.Overexpression of ARMC10 rescued mitochondrial dysfunction induced by OGD/R injury, reduced neuronal apoptosis and exerted neuroprotective effects after IS.Interestingly, Wnt/β-catenin signalling pathway agonists had similar mitochondrial protective effects compared with overexpression of ARMC10.Recent studies have shown that activation of the Wnt/β-catenin signalling pathway improves neurological function by inhibiting mitochondrial oxidative stress, increasing mitochondrial membrane potential, inhibiting apoptosis and promoting mitochondrial biogenesis in Parkinson's disease rats.36Consistent with previous studies, we also found that the protective effect of ARMC10 against brain I/R injury was regulated by the Wnt/β-catenin signalling pathway.Our results suggested that the Wnt/β-catenin signalling pathway may serve as a promising candidate for neuroprotective therapy.There are some limitations to this study.First, the clinical sample size is small and there may be some confounding factors, which may cause bias in the statistical results.It is necessary to further expand the sample size to investigate the changing pattern of ARMC10 to provide data for clinical diagnosis and prognosis.Secondly, this study only investigated the mechanism by which ARMC10 affects mitochondrial dynamics, mitochondrial function and neuronal apoptosis in ischaemic stroke in a cellular model, but lacked the corroboration of in vivo studies.The results of cellular experiments need to be further validated by constructing a mouse MCAO model.